coni-cat翻译
西
南
大
学
课
程
考
核
西南大学
生物技术学院
《
Molecular Biology
》课程试题
【
A
】卷
201
8
~
2
019
学
年
第
1
学
期
考试时间
120
分钟
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期末
考试
本科
考核方式
开卷笔试
学生类别
生物技术
人数
98
05
级
十
学
号
适用专业或科类
题号
得分
签名
一
年级
六
密
二
三
四
五
七
八
九
合计
姓
名
阅卷须知:阅卷用红色墨水笔书写,得分用阿拉伯数字写在 每小题题号前,用正分表示,不得分则在题号前
写
0
;大题得分登录在对应的分数框内 ;统一命题的课程应集体阅卷,流水作业;阅卷后要进行复核,发现漏
评、漏记或总分统计错误应及时更 正;对评定分数或统分记录进行修改时,修改人必须签名。
班
封
特别提醒:学生必须遵守课程考核纪律,违规者将受到严肃处理。
线
I Define the meaning of these following terms (30 points, 3 pts per term)
1.
melting temperature (Tm)
2.
C value paradox
3.
exon
4.
semi-conservation replication
5.
cDNA library
6.
gel shift assay
命题教师:
教研室或系负责人:
主管院长:
年
月
日
第
1
页
共
5
页
学
院
专
业
年
级
《
Molecular
Biology
》课程试题
【
A
】卷
7.
promoter
8.
open reading frame (ORF)
9.
gene family
10.
expressed sequence tag (EST)
II Fill in blank (10 points, 1 pts per blank)
1.
The simplest cloning vector at least contains
,
and ori elements.
2. The diagram below shows two RNA molecules. Molecule I is processed to give molecule II.
Answer the following questions about these two molecules:
Where does this conversion happen? ____________________________
What type of RNA molecule is molecule I? _________________________
What type of RNA molecule is molecule II? _________________________
Name the regions/structures indicated by the arrows:
A = _________________
B = _________________
C = _________________
D = _________________
E = _________________
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西
南
大
学
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考
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试题
【
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)
密
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III Select the right answer (20 points, 2 pts per question;
Select the
best answer
for
each question.
)
1. The bond between a phosphate group and the ribose sugar groups in RNA is called which of the
following?
(
)
(A) Phosphodiester bond
(B) Glycosidic bond
(C) Peptide bond
(D) Amide bond
2.
Which enzyme does not play a role in DNA replication?
(
)
(A) DNA polymerase
(B) Helicase
(C) Single-stranded binding protein
(D) Guanyl transferase
3. Splicing is the process that does which of the following? (
)
(A) Remove introns and conserve exons
(B) Remove exons and conserve introns
(C) Remove mutated regions of primary transcript RNA
(D) Add multiple adenosine bases to the end of a primary RNA transcript
4. The ideal spacing between the -10 and -35
regions of a prokaryotic promoter is: (
) bp
.
(A) 14
(B) 15
(C) 16
(D) 17
5. The 5' capping process creates what type of linkage? (
)
(A) 5'-3'
(B) 3'-5'
(C) 5'-5'
(D) 3'-3'
6. Codons that code for the same amino acid are called: (
)
(A) Synonyms
(B) Similar
(C) Degenerates
(D) Complements
7. Which of the following is not a characteristic of a tRNA molecule? (
)
(A) D arm
(B) Beta arm
(C) T arm
(D) Anti-codon arm
8. What signals the end of transcription? (
)
(A) Stop codon
(B) Terminator
(C) The end of the DNA chain
(D) RNA polymerase runs out
9. The start codon is: (
)
(A) AUU
(B) UAA
(C) AUG
(D) UAG
10. Which of the following is the sequence of the -10 region of prokaryotic promoters? (
)
(A) TATAAT
(B) TTGACA
(C) TA
TA
(D) CAA
IV Analytical questions (30 points, 6 pts per question)
1. Describe briefly the attenuation of the
trp
operon.
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《
Molecular
Biology
》课程试题
【
A
】卷
2. Describe the principle
of Sanger’s enzymic method for DNA sequencing.
3. What are the basic features of DNA double helix deduced by Watson and Crick?
4. Please draw a schematic structure of TTP in the following space. You may write just the letter
base.
5. On the following agarose gel with marker DNA as indicated:
a)
Place a plus (+) sign on the positive pole of the gel and a minus (
–
) sign on the negative pole.
b)
Draw the band or bands that you would see if you digested a 3 kb plasmid with EcoRI and ran it to the
left of the marker.
Assume the plasmid has a MCS with the following restriction sites:
BamHI, EcoRI,
EcoRV
, HindIII, and KpnI.
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西
南
大
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试题
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)
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————————— —————
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6 kb
4 kb
2 kb
0.5 k
b
V Discuss the following topic (10 points)
Describe your understanding of Central Dogma in detail.
当我被上帝造出来时,上帝问我想在人间当一个怎样的人,我不假思索的说,我要做一个伟大的世人皆知的人。于是,我降临在了人间。
我出生在一个官僚知识分子之 家,
父亲在朝中做官,
精读诗书,
母亲知书答礼,
温柔体贴,父母给我去了一 个好听的名字:李清照。
小时侯,受父母影响的我饱读诗书,聪明伶俐,在朝中享有
“
神童
”
的称号。小
时候的我天真活泼,才思敏捷,小河畔,花丛边撒满了我 的诗我的笑,无可置疑,
小时侯的我快乐无虑。
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