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cat翻译分子生物学试卷(英文)

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2021-01-20 03:40
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coni-cat翻译

2021年1月20日发(作者:aggression)
西






















西南大学

生物技术学院


Molecular Biology
》课程试题


A
】卷

201
8

2
019




1



考试时间

120
分钟

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期末

考试

本科

考核方式

开卷笔试

学生类别

生物技术

人数

98
05









适用专业或科类

题号

得分

签名





年级


































合计







阅卷须知:阅卷用红色墨水笔书写,得分用阿拉伯数字写在 每小题题号前,用正分表示,不得分则在题号前

0
;大题得分登录在对应的分数框内 ;统一命题的课程应集体阅卷,流水作业;阅卷后要进行复核,发现漏
评、漏记或总分统计错误应及时更 正;对评定分数或统分记录进行修改时,修改人必须签名。







特别提醒:学生必须遵守课程考核纪律,违规者将受到严肃处理。

线
I Define the meaning of these following terms (30 points, 3 pts per term)
1.

melting temperature (Tm)


2.

C value paradox


3.

exon


4.

semi-conservation replication


5.

cDNA library


6.

gel shift assay



命题教师:
















教研室或系负责人:











主管院长:













































































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Molecular
Biology

》课程试题


A
】卷

7.

promoter


8.

open reading frame (ORF)


9.

gene family


10.

expressed sequence tag (EST)


II Fill in blank (10 points, 1 pts per blank)
1.
The simplest cloning vector at least contains













,










and ori elements.
2. The diagram below shows two RNA molecules. Molecule I is processed to give molecule II.

Answer the following questions about these two molecules:
Where does this conversion happen? ____________________________
What type of RNA molecule is molecule I? _________________________
What type of RNA molecule is molecule II? _________________________
Name the regions/structures indicated by the arrows:
A = _________________


B = _________________


C = _________________

D = _________________

E = _________________






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(
试题


A
】卷
)


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III Select the right answer (20 points, 2 pts per question;
Select the
best answer
for

each question.
)
1. The bond between a phosphate group and the ribose sugar groups in RNA is called which of the
following?

(




)
(A) Phosphodiester bond

(B) Glycosidic bond

(C) Peptide bond









(D) Amide bond

2.

Which enzyme does not play a role in DNA replication?

(




)

(A) DNA polymerase




(B) Helicase

(C) Single-stranded binding protein

(D) Guanyl transferase

3. Splicing is the process that does which of the following? (




)
(A) Remove introns and conserve exons

(B) Remove exons and conserve introns

(C) Remove mutated regions of primary transcript RNA

(D) Add multiple adenosine bases to the end of a primary RNA transcript

4. The ideal spacing between the -10 and -35

regions of a prokaryotic promoter is: (




) bp
.

(A) 14

(B) 15

(C) 16


(D) 17

5. The 5' capping process creates what type of linkage? (




)
(A) 5'-3'


(B) 3'-5'

(C) 5'-5'


(D) 3'-3'

6. Codons that code for the same amino acid are called: (




)
(A) Synonyms


(B) Similar

(C) Degenerates


(D) Complements

7. Which of the following is not a characteristic of a tRNA molecule? (




)
(A) D arm



(B) Beta arm

(C) T arm



(D) Anti-codon arm
8. What signals the end of transcription? (




)
(A) Stop codon





(B) Terminator

(C) The end of the DNA chain


(D) RNA polymerase runs out

9. The start codon is: (




)
(A) AUU



(B) UAA

(C) AUG



(D) UAG

10. Which of the following is the sequence of the -10 region of prokaryotic promoters? (




)
(A) TATAAT


(B) TTGACA

(C) TA
TA



(D) CAA

IV Analytical questions (30 points, 6 pts per question)
1. Describe briefly the attenuation of the
trp
operon.



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Molecular
Biology

》课程试题


A
】卷







2. Describe the principle
of Sanger’s enzymic method for DNA sequencing.








3. What are the basic features of DNA double helix deduced by Watson and Crick?







4. Please draw a schematic structure of TTP in the following space. You may write just the letter
base.







5. On the following agarose gel with marker DNA as indicated:
a)

Place a plus (+) sign on the positive pole of the gel and a minus (

) sign on the negative pole.
b)

Draw the band or bands that you would see if you digested a 3 kb plasmid with EcoRI and ran it to the
left of the marker.

Assume the plasmid has a MCS with the following restriction sites:

BamHI, EcoRI,
EcoRV
, HindIII, and KpnI.



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(
试题


A
】卷
)


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6 kb
4 kb
2 kb
0.5 k
b

V Discuss the following topic (10 points)
Describe your understanding of Central Dogma in detail.








当我被上帝造出来时,上帝问我想在人间当一个怎样的人,我不假思索的说,我要做一个伟大的世人皆知的人。于是,我降临在了人间。

我出生在一个官僚知识分子之 家,
父亲在朝中做官,
精读诗书,
母亲知书答礼,
温柔体贴,父母给我去了一 个好听的名字:李清照。

小时侯,受父母影响的我饱读诗书,聪明伶俐,在朝中享有

神童

的称号。小
时候的我天真活泼,才思敏捷,小河畔,花丛边撒满了我 的诗我的笑,无可置疑,
小时侯的我快乐无虑。











































































































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coni-cat翻译


coni-cat翻译


coni-cat翻译


coni-cat翻译


coni-cat翻译


coni-cat翻译


coni-cat翻译


coni-cat翻译



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