snf-doughnut
各种激发光和吸收光波长
现在发现一个更全的有关激发光和发射光的波长图谱。这对
CONFOCAL
去做多重染色时是必
不可少的参考资料。
全部文件
Excitation and Emission Wavelengths of Fluorophores
Tavi's
(from )
Fluorophore
Absorption
Emission
Other info
1,5 IAEDANS
336
490
1,8-ANS
372
480
4-Methylumbelliferone
385
502
5-carboxy-2,7-dichlorofluorescein
504
529
5-Carboxyfluorescein (5-FAM)
492
518
5-Carboxynapthofluorescein (pH 10)
512/598
563/668
Ratio Dye, pH
5-Carboxytetramethylrhodamine (5-TAMRA)
542
568
5-FAM (5-Carboxyfluorescein)
492
518
5-HAT (Hydroxy Tryptamine)
370-415
520-540
5-Hydroxy Tryptamine (HAT)
370-415
520-540
5-ROX (carboxy-X- rhodamine)
578
604
567
591
5-TAMRA (5-Carboxytetramethylrhodamine)
548
552
542
568
6-Carboxyrhodamine 6G
518
543
6-CR 6G
518
543
6-JOE
520
548
7-Amino-4-methylcoumarin
351
430
7-Aminoactinomycin D (7-AAD)
546
647
7-Hydroxy-4-methylcoumarin
360
449, 455
9-Amino-6-chloro-2-methoxyacridine
412, 430
471, 474
ABQ
344
445
Acid Fuchsin
540
630
ACMA (9-Amino-6-chloro-2-methoxyacridine)
412, 430
471, 474
Acridine Orange + DNA
502
526
Acridine Orange + RNA
460
650
Acridine Orange, both DNA & RNA
440-480
520-650
Acridine Red
455-600
560-680
Acridine Yellow
470
550
Acriflavin
436
520
Acriflavin Feulgen SITSA
355-425
460
Aequorin (Photoprotein)
466
Photoprotein
AFPs
-
AutoFluorescent
Protein
-
(Quantum
Biotechnologies) see sgGFP
, sgBFP
Alexa Fluor 350
346
442
342
441
Alexa Fluor 430
Alexa Fluor 488
Alexa Fluor 532
Alexa Fluor 546
Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 633
Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
Alizarin Complexon
Alizarin Red
Allophycocyanin (APC)
AMC, AMCA-S
AMCA (Aminomethylcoumarin)
AMCA-X
Aminoactinomycin D
Aminocoumarin
Aminomethylcoumarin (AMCA)
Anilin Blue
Anthrocyl stearate
APC (Allophycocyanin)
APC-Cy7
APTRA-BTC = Ratio Dye, Zn2+
APTS
Astrazon Brilliant Red 4G
Astrazon Orange R
Astrazon Red 6B
Astrazon Yellow 7 GLL
Atabrine
ATTO- TAG CBQCA
ATTO-TAG FQ
Auramine
Aurophosphine G
Aurophosphine
BAO 9(Bisaminophenyloxadiazole)
BCECF (high pH)
BCECF (low pH)
Berberine Sulphate
Beta Lactamase
431
495, 492
531, 532
556, 557
577, 578
590, 594
632
647
668
679
530-560,
580
530-560
630, 645
345
345
347
353
555
346
350
345
347
360-381
630, 645
625-650
466/380
424
500
470
520
450
436
465
486
460
450
450-490
365
492, 503
482
430
409
540
519, 520
553, 554
572, 573
603
617, 618
650
666
698
702
580,
624-645
580
655, 660
445
425
444
442
655
442
445
425
444
600
446
655, 660
755
520/530
505
585
540
595
480
490
560
591
550
580
515
395
520,528
520
550
447, 520
Ratio Dye, Zn2+
BFP blue shifted GFP (Y66H)
Blue Fluorescent Protein
BFP/GFP FRET
Bimane
Bisbenzamide
Bisbenzimide (Hoechst)
bis-BTC = Ratio Dye, Zn2+
Blancophor FFG
Blancophor SV
BOBO-1
BOBO-3
Bodipy 492/515
Bodipy 493/503
Bodipy 500/510
Bodipy 505/515
Bodipy 530/550
Bodipy 542/563
Bodipy 558/568
Bodipy 564/570
Bodipy 576/589
Bodipy 581/591
Bodipy 630/650-X
Bodipy 650/665-X
Bodipy 665/676
Bodipy Fl
Bodipy FL ATP
Bodipy Fl- Ceramide
Bodipy R6G SE
Bodipy TMR
Bodipy TMR-X conjugate
Bodipy TMR-X, SE
Bodipy TR
Bodipy TR ATP
Bodipy TR-X SE
BO-PRO-1
BO-PRO-3
Brilliant Sulphoflavin FF
BTC - Ratio Dye Ca2+
BTC-5N - atio Dye, Zn2+
Calcein
Calcein Blue
Calcium Crimson
Calcium Green
Calcium Green-1 Ca2+ Dye
381,
382,
445,
447,
blue shifted GFP (Y66H)
383
448
Blue Fluorescent Protein
398
490
360
461
360
461
455/405
529/505
Ratio Dye, Zn2+
390
470
370
435
462
481
570
602
490
515
533
549
509
515
502
510
528
547
543
563
558
569
564
570
579
590
584
592
625
642
647
665
605
676
504, 505
511, 513
505
514
505
511
528
547
542
574
544
573
544
570
589
617
591
620
588
616
462
481
544
570
430
520
464/401
533/529
Ratio Dye Ca2+
459/417
517/532
Ratio Dye, Zn2+
494
517
373
440
588, 589
611, 615
501, 506
531
506
531
Ca2+ Dye
Calcium Green-2 Ca2+
Calcium Green-5N Ca2+
Calcium Green-C18 Ca2+
Calcium Orange
Calcofluor White
Carboxy-X-rhodamine (5-ROX)
Cascade Blue
506/503
506
509
549
Cascade Yellow
Catecholamine
CCF2 (GeneBlazer)
CFDA
CFP - Cyan Fluorescent Protein
CFP/YFP FRET
Chlorophyll
Chromomycin A
Chromomycin A
CL-NERF (Ratio Dye, pH)
CMFDA
Coelenterazine Ca2+ Dye, bioluminescence
Coelenterazine cp (Ca2+ Dye,)
Coelenterazine f
Coelenterazine fcp
Coelenterazine h
Coelenterazine hcp
Coelenterazine ip
Coelenterazine n
Coelenterazine O
Coumarin Phalloidin
C-phycocyanine
385,
405
576
377
398
399
399
550
400
552
410
470
494
520
430,
433,
474,
475,
Cyan Fluorescent Protein
436, (453)
476, (501)
480
650
436-460
470
445
575
504/514
540
Ratio Dye, pH
494
520
(429)
465
Ca2+
Dye,
bioluminescence,
native molecule
(430)
442
Ca2+
Dye,
bioluminescence
(437)
473
Ca2+
Dye,
bioluminescence
452
Ca2+
Dye,
bioluminescence
(437)
464
Ca2+
Dye,
bioluminescence
(433)
444
Ca2+
Dye,
bioluminescence
441
Ca2+
Dye,
bioluminescence
(431)
467
Ca2+
Dye,
bioluminescence
460
575
387
470
536
Ca2+
532
Ca2+
530
Ca2+
575
576
395,
437,
440,
445
601
420
423
snf-doughnut
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snf-doughnut
snf-doughnut
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snf-doughnut
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