2021年1月23日发(作者：capability)
PCR
实验室建设基本要求（中英对照）





PCR
实验室依据所使用的方法可分为试剂准备、标本制备、扩增、产物分析等< br>三或四个区域。只是使用实时荧光
PCR
仪、
HIV
病毒载量测定仪的
PCR
实验
室，有上述前面三个区域即可。各区域应完全独立分隔，不应有任何的空气 直
通。

Based on the methods used by PCR lab, the PCR laboratory can be
divided into three or four rooms or areas for (i) reagent preparation, (ii)
sample preparation, (iii) amplification area, and (iv) product analysis
area. If the PCR laboratory only uses real- time fluorescence PCR
instruments and /or instruments for HIV virus load, the PCR lab may
have only the first three rooms or areas (reagent preparation, sample
preparation, amplification). The rooms or areas of the PCR lab should
be independently separated, and there is no air flow through the
separated rooms or areas.


产物分析区或三个区域的最后一个区域（即扩增及产物分析区）， 空气流向应
由室外向室内，可通过在室内设置通风橱、排风扇或其他排风系统达到空气流
由室外 向室内流动的要求。

For PCR product analysis area or last area of three areas (area for
amplification and product analysis), direction of air flow shall be from
outside to inside of the area by setting up fume hood, exhaust fan or
other exhaust system in the area.


PCR
实验室各区域仪器设备配备通常如下：

The equipment configuration for PCR labs are as follows: