脊髓损伤打击器--NYU 脊椎冲击损伤仪

Cd

），脊髓压缩时间

(Ct)

，

脊髓压缩率

(Cr)

。

这些撞击参数本身是相关的，

同时与其它的脊髓损伤数值如组织

Na

，

K

浓

度，运动恢复

locomotor

recovery(BBB

评分

)< /p>

也是相关的。

大小鼠的撞击都需要一个固定系统

(Clamping

systems)

。同时还需要一个

CS- tie

NYU

脊椎冲击损伤仪

由

NYU(W.M.

Keck

Center

for

Collaborative

Neuroscience,

Rutgers

the

State

Univers

ity of New Jersey)

出品的

rat/mouse

脊椎冲击损伤仪 ，在全世界很受歡迎。其

產品目前有分兩款，一是

model I

，可以接電腦，二是

model II

，可以不接電腦，這兩款

大

/

小鼠均 可以使用，

且發表論文也非常多，

在脊椎損傷研究

,

有超過

50%

的論文都是 用

NYU

impactor

來發表。

Overview

The MASCIS Impactor is a device designed to deliver graded reproducible spinal

cord contusions in rats.

Developed

over

ten

years

ago, the

Impactor

is

part

of

a

well-defined

rodent

spinal cord injury model that is used in over 100 laboratories around the world.

Most

of the recommended procedures for the Impactor are based on experience

with

the

model

and

work

done by

the

Multicenter

Animal

Spinal

Cord

Injury

Study

(MASCIS).

The

Impactor

is

now

in

its

third

generation

with

many

improvements over previous models.

Introduction

The MASCIS Impactor

, formerly called the NYU Impactor

, was developed in 1991

by Drs. John Gruner

, Carl Mason, and

Wise Young. It is now used in laboratories

throughout the world in their spinal cord injury studies. The device precisely

measures the movement of a 10-gram rod dropped

12.5, 25.0, or 50.0 mm onto

the

T9-10

spinal

cord

exposed

by

laminectomy.

In

addition,

the

device

measures

movement

of

the

spinal

column

at

the

impact

site,

displays

the

trajectory

of

the

falling

rod,

and

measures the

impact

velocity

(ImpV),

cord

compression distance (Cd), cord compression time (Ct), and cord compression

rate (Cr). These impact parameters correlate with each other and spinal cord

lesion volumes (estimated from tissue Na and K concentrations) and locomotor

recovery (BBB scores).

In addition, NYU Impactor allows the end user to measure below parameter:

Impact velocity (ImpV)

cord compression distance (Cd)

cord compression time (Ct)

cord compression rate (Cr)

Clamping systems are

available for both rat and mice. A

clamping system

is

necessary for NYU Impactor

. When Using Clamping system, a CS-tie device

is

needed.

Size

The MASCIS Impactor is 17” high x 12” deep and 10” wide.

It weighs approximately 11 pounds.

The MASCIS Impactor is a device designed to deliver graded reproducible spinal

cord contusions in rats. Developed over ten years ago, the Impactor is part of a

well-defined

rodent

spinal

cord

injury

model

that

is

used

in

over

100

laboratories

around

the

world.

In

addition,

more

than

50%

of

recent

publications on spinal cord injury research used the MASCIS Impactor

. Most of

the recommended procedures for the Impactor are based on experience

with

the model and work done by the Multicenter Animal Spinal Cord Injury Study

(MASCIS).

The

Impactor

is

now

in

its

third

generation

with

many

improvements

over

previous

models.

It

is

available

in

a

model

with

data

recording

capability

(description

and

picture

links)

which

requires

a

Pentium

computer

.

It

is

also

available in a bas

ic model (The Rutgers Impactor) which only does the impact,

not the recording of data.

Clamping systems are available for both rat and mouse. A clamping system is

necessary for the Rutgers basic model and serves as a functional enhancement

for the MASCIS model. When using a clamping system with the MASCIS model,

a CS-tie device also is needed.

1. Serial Changes in Bladder

, Locomotion, and in Levels of Neurotrophic Factors

in Rats with Spinal Cord Contusion.

Hyun JK, Lee YI, Son YJ, Park JS.

J Neurotrauma. 2009 Feb 9.

Dankook

University

College

of

Medicine,

Rehabilitation

Medicine,

San

16-5

Anseo-dong,

Cheonan,

Korea,

Republic

of,

330-714,

82-41-550-6640,

82-41-551-7062; rhhyun@

.

The aims of this study were to evaluate the evolution of the neurogenic bladder

after spinal cord contusion, and to correlate changes in bladder function with

locomotor

function

and

levels

of

neurotrophic

factors.

The

MASCIS

impactor

was used to cause a mild contusion injury of the lower thoracic spinal cord of

Sprague-Dawley rats.

Rats were divided into four groups according

to

the length

of

time

from

injury

to

sacrifice,

at

4,

14,

28,

and

56

days

after

injury.

Gait

analysis was performed each week, and urodynamic study was performed just

before

sacrifice.

Basso,

Beattie,

and

Bresnahan

(BBB)

and

coupling

scores

showed gradual recovery, as did the urinary voiding pattern and bladder volume;

some

parameters

of

micturition

reached

normal

ranges.

Brain-derived

neurotrophic

factor

(BDNF)

levels

in

the

spinal

cord,

as

detected

by

enzyme-linked

immunosorbent

assay,

decreased

with

time,

whereas

neurotrophin-3

(NT-3)

levels

remained

unchanged.

The

micturition

pattern,

bladder volume and locomotor function continued to recover during the time of

observation;

BDNF

levels

in

the

spinal

cord

and

bladder

were

inversely

correlated with BBB scores and the restoration of bladder volume. We conclude

that urodynamic changes in the bladder correlate with locomotion recovery but

not with the levels of BDNF or NT3 after modified mild contusion injury in rats.

2: A re- assessment of minocycline

as a neuroprotective agent

in a rat spinal

cord contusion model.

Pinzon A, Marcillo A, Quintana A, Stamler S, Bunge MB, Bramlett HM, Dietrich

WD.

Brain Res. 2008 Dec 3;1243:146-51. Epub 2008 Sep 24.

3:

The

role

of

cation-dependent

chloride

transporters

in

neuropathic

pain

following spinal cord injury.

Cramer SW, Baggott C, Cain J, Tilghman J, Allcock B, Miranpuri G, Rajpal S, Sun

D, Resnick D.

Mol Pain. 2008 Sep 17;4:36.

4: Novel combination strategies to repair the injured mammalian spinal cord.

Bunge MB.

J Spinal Cord Med. 2008;31(3):262-9. Review.

5: A re-assessment of erythropoietin as a neuroprotective agent following rat

spinal cord compression or contusion injury.

Pinzon A, Marcillo A, Pabon D, Bramlett HM, Bunge MB, Dietrich WD.

Exp Neurol. 2008 Sep;213(1):129-36. Epub 2008 Jul 14.

6:

B1

and

TRPV-1

receptor

genes

and

their

relationship

to

hyperalgesia

following spinal cord injury.

DomBourian MG, Turner NA, Gerovac TA, Vemuganti R, Miranpuri GS, Tü

reyen K,

Satriotomo I, Miletic V, Resnick DK.

Spine. 2006 Nov 15;31(24):2778-82.

7:

Endothelial

cell

loss

is

not

a

major

cause

of

neuronal

and

glial

cell

death

following contusion injury of the spinal cord.

Casella GT, Bunge MB, Wood PM.

Exp Neurol. 2006 Nov;202(1):8-20. Epub 2006 Jul 26.

8:

Recovery

of

function

following

grafting

of

human

bone

marrow-derived

stromal cells into the injured spinal cord.

Himes BT, Neuhuber B, Coleman C, Kushner R, Swanger SA,

Kopen GC, Wagner

J, Shumsky JS, Fischer I.

Neurorehabil Neural Repair. 2006 Jun;20(2):278-96.

9: Mechanical and cold allodynia in a rat spinal cord contusion model.

Yoon YW, Dong H, Arends JJ, Jacquin MF

.

Somatosens Mot Res. 2004 Mar;21(1):25-31.

10: Spinal cord contusion models.

Young W.

Prog Brain Res. 2002;137:231-55. Review.