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植物表达载体 Pcambia1302 序列图谱

作者:高考题库网
来源:https://www.bjmy2z.cn/gaokao
2021-02-27 22:07
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2021年2月27日发(作者:micu)


载体质粒图谱和多克隆位点信息






载体简介



载体序列



LOCUS AF234298 10549 bp DNA circular SYN 24-APR-2000


DEFINITION Binary vector pCAMBIA-1302, complete sequence.


ACCESSION AF234298


VERSION AF234298.1 GI:7638073


KEYWORDS .


SOURCE Binary vector pCAMBIA-1302


ORGANISM Binary vector pCAMBIA-1302


other sequences; artificial sequences; vectors.


REFERENCE 1 (sites)


AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.


TITLE The small, versatile pPZP family of Agrobacterium binary vectors


for plant transformation


JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)


PUBMED 7919218


REFERENCE 2 (bases 1 to 10549)


AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,


Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,


Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,


Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.


TITLE A comprehensive set of modular vectors for advanced manipulations


and efficient transformation of plants


JOURNAL Unpublished


REMARK Full description of constructs


REFERENCE 3 (bases 1 to 10549)


AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,


Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,


Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,


Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.


TITLE Direct Submission


JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /


GPO Box 3200, Canberra, ACT 2601, Australia


FEATURES Location/Qualifiers


source 1..10549


/organism=


/mol_type=


/db_xref=


CDS 2..757


/note=


/codon_start=1


/product=


/protein_id=


/db_xref=


/translation=


KLTLK FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQE RTI


FFKDDGNYKTRAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK


QKNGIKANFKTRHNIEDG GVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK


RDHMVLLEFVTAAGITHGMDELYKASHHHHHHV


misc_feature 786..1038


/note=


misc_feature 1076..1101


/note=


misc_feature complement(2142..3142)


/note=


rep_origin complement(3735..4735)


/note=


misc_feature complement(5145..5405)


/note=


rep_origin complement(5545..5825)


/note=


CDS complement(6116..6910)


/note=


pIG121Hm


/codon_start=1


/product=


/protein_id=


/db_xref=


/translation=


MTDSR YKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCS EEY


EDEQSPEKIIELYA ECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE


EDTPFKDPRELYDFLKTE KPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW


YDIAFCVRSIREDIGEEQ YVELFFDLLGIKPDWEKIKYYILLDELF


misc_feature 7335..7360


/note=


misc_feature 7427..7635


/note=


CDS complement(7651..8676)


/note=


/codon_start=1


/product=


/protein_id=


/db_xref=


/translation=


GYVLR VNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVT LQD


LPETELPAVLQPVA EAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY


HWQTVMDDTVSASVAQAL DELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS


EAMFGDSQYEVANIFFWR PWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ


SLVDGNFDDAAWAQGRCD AIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS


TRPRAKK


promoter complement(8712..9492)


/note=


promoter 9640..9694


/note=


CDS 9722..9955


/codon_start=1


/product=


/protein_id=


/db_xref=


/translation=


RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC


misc_feature 9736..9792


/note=


promoter 10006..10543


/note=


ORIGIN


1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt


61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga


121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc


181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga


241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag


301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg


361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat


421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa


481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt


541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc


601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga


661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact


721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc


781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg


841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat


901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat


961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat


1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa


1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta


1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact


1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct


1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt


1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac


1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc


1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc

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