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载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS
AF234298 10549 bp DNA
circular SYN 24-APR-2000
DEFINITION
Binary vector pCAMBIA-1302, complete sequence.
ACCESSION AF234298
VERSION
AF234298.1 GI:7638073
KEYWORDS .
SOURCE Binary vector pCAMBIA-1302
ORGANISM Binary vector pCAMBIA-1302
other sequences; artificial
sequences; vectors.
REFERENCE 1
(sites)
AUTHORS Hajdukiewicz,P.,
Svab,Z. and Maliga,P.
TITLE The
small, versatile pPZP family of Agrobacterium
binary vectors
for plant
transformation
JOURNAL Plant Mol.
Biol. 25 (6), 989-994 (1994)
PUBMED
7919218
REFERENCE 2 (bases 1 to
10549)
AUTHORS Roberts,C.,
Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S.,
Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I.,
Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and
Jefferson,R.A.
TITLE A
comprehensive set of modular vectors for advanced
manipulations
and efficient
transformation of plants
JOURNAL
Unpublished
REMARK Full
description of constructs
REFERENCE 3
(bases 1 to 10549)
AUTHORS
Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A.,
Yang,W.,
Nugrohu,S.,
Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N.,
Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and
Jefferson,R.A.
TITLE Direct
Submission
JOURNAL Submitted
(15-FEB-2000) CAMBIA, Clunies Ross St, Black
Mountain /
GPO Box 3200,
Canberra, ACT 2601, Australia
FEATURES
Location/Qualifiers
source
1..10549
/organism=
/mol_type=
/db_xref=
CDS 2..757
/note=
/codon_start=1
/product=
/protein_id=
/db_xref=
/translation=
KLTLK
FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQE
RTI
FFKDDGNYKTRAEV
KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK
QKNGIKANFKTRHNIEDG
GVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK
RDHMVLLEFVTAAGITHGMDELYKASHHHHHHV
misc_feature 786..1038
/note=
misc_feature 1076..1101
/note=
misc_feature complement(2142..3142)
/note=
rep_origin
complement(3735..4735)
/note=
misc_feature
complement(5145..5405)
/note=
rep_origin
complement(5545..5825)
/note=
CDS
complement(6116..6910)
/note=
pIG121Hm
/codon_start=1
/product=
/protein_id=
/db_xref=
/translation=
MTDSR
YKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCS
EEY
EDEQSPEKIIELYA
ECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTE
KPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQ
YVELFFDLLGIKPDWEKIKYYILLDELF
misc_feature 7335..7360
/note=
misc_feature 7427..7635
/note=
CDS complement(7651..8676)
/note=
/codon_start=1
/product=
/protein_id=
/db_xref=
/translation=
GYVLR
VNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVT
LQD
LPETELPAVLQPVA
EAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQAL
DELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWR
PWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCD
AIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKK
promoter complement(8712..9492)
/note=
promoter 9640..9694
/note=
CDS 9722..9955
/codon_start=1
/product=
/protein_id=
/db_xref=
/translation=
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC
misc_feature 9736..9792
/note=
promoter 10006..10543
/note=
ORIGIN
1
catggtagat ctgactagta aaggagaaga acttttcact
ggagttgtcc caattcttgt
61
tgaattagat ggtgatgtta atgggcacaa attttctgtc
agtggagagg gtgaaggtga
121
tgcaacatac ggaaaactta cccttaaatt tatttgcact
actggaaaac tacctgttcc
181
gtggccaaca cttgtcacta ctttctctta tggtgttcaa
tgcttttcaa gatacccaga
241
tcatatgaag cggcacgact tcttcaagag cgccatgcct
gagggatacg tgcaggagag
301
gaccatcttc ttcaaggacg acgggaacta caagacacgt
gctgaagtca agtttgaggg
361
agacaccctc gtcaacagga tcgagcttaa gggaatcgat
ttcaaggagg acggaaacat
421
cctcggccac aagttggaat acaactacaa ctcccacaac
gtatacatca tggccgacaa
481
gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac
aacatcgaag acggcggcgt
541
gcaactcgct gatcattatc aacaaaatac tccaattggc
gatggccctg tccttttacc
601
agacaaccat tacctgtcca cacaatctgc cctttcgaaa
gatcccaacg aaaagagaga
661
ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
acacatggca tggatgaact
721
atacaaagct agccaccacc accaccacca cgtgtgaatt
ggtgaccagc tcgaatttcc
781
ccgatcgttc aaacatttgg caataaagtt tcttaagatt
gaatcctgtt gccggtcttg
841
cgatgattat catataattt ctgttgaatt acgttaagca
tgtaataatt aacatgtaat
901
gcatgacgtt atttatgaga tgggttttta tgattagagt
cccgcaatta tacatttaat
961
acgcgataga aaacaaaata tagcgcgcaa actaggataa
attatcgcgc gcggtgtcat
1021
ctatgttact agatcgggaa ttaaactatc agtgtttgac
aggatatatt ggcgggtaaa
1081
cctaagagaa aagagcgttt attagaataa cggatattta
aaagggcgtg aaaaggttta
1141
tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
cccctcggga tcaaagtact
1201
ttgatccaac ccctccgctg ctatagtgca gtcggcttct
gacgttcagt gcagccgtct
1261
tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
aggctgccgc cctgcccttt
1321
tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
agaatacttg cgactagaac
1381
cggagacatt acgccatgaa caagagcgcc gccgctggcc
tgctgggcta tgcccgcgtc
1441
agcaccgacg accaggactt gaccaaccaa cgggccgaac
tgcacgcggc cggctgcacc
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