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常用酿酒酵母菌株基因型

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2021-03-01 10:42
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Commonly used strains


This page describes some of the most commonly used yeast lab strains. Much of the information is taken from


F.


Sherman (2002)


Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background


information include:


?



?



Mortimer and Johnston


(1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C


van Dijken et al.


(2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly


used lab strains


?



Winzeler et al.


(2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic


identity between common lab strains


S288C


Genotype:



MAT


α



SUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6



Notes:


Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form


pseudohyphae. In addition, since it has a mutated copy of


HAP1


, it is not a good strain for mitochondrial studies. It has


an allelic variant of


MIP1


which increases petite frequency. S288C strains are


gal


2- and they do not use galactose


anaerobically.


The S288C genome was recently resequenced at the


Sanger Institute


.


References:



Mortimer and Johnston


(1986) Genetics 113:35-43.


Sources:



ATCC:204508



BY4743


Genotype:



MAT


a/α



his3


Δ


1/his3


Δ


1 leu2


Δ


0/leu2

< br>Δ


0 LYS2/lys2


Δ


0 met15


Δ


0/MET15 ura3


Δ


0/ura3


Δ


0



Notes:


Strain used in the


systematic deletion project


, generated from a cross between BY4741 and BY4742, which


are derived from S288C. As S288c, these strains have an allelic variant of


MIP1


which increases petite frequency.


See Brachmann et al. reference for details.


References:



Brachmann et al.


(1998) Yeast 14:115-32.


Sources:



Biosystems:YSC1050



FY4


Genotype:



MAT


a


Notes:


Derived from S288C.


References:



Winston et al.


(1995) Yeast 11:53-55.


Brachmann et al.


(1998) Yeast 14:115-32.


FY1679


Genotype:



MAT


a/α



ura3-52/ura3-52 trp1


Δ


63/TRP1 leu2


Δ


1/LEU2 his3


Δ


200/HIS3 GAL2/GAL



Notes:


Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.


References:



Winston et al.


(1995) Yeast 11:53-55.


Sources:



EUROSCARF:10000D



AB972


Genotype:



MAT


α


X2180-1B trp1


0


[rho


0


]



Notes:


Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an


ethidium bromide-induced rho- derivative of the strain X2180-1B-


trp1


.


References:



Olson MV et al.


(1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.


Sources:



ATCC:204511



A364A


Genotype:



MAT


a


ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIO



Notes:


Used in the systematic sequencing project, the sequence stored in SGD.


References:



Hartwell


(1967) J. Bacteriol. 93:1662-1670.


Sources:



ATCC:208526



XJ24-24a


Genotype:



MAT


a


ho HMa HMα ade6 arg4


-17 trp1-1 tyr7-1 MAL2



Notes:


Derived from, but not isogenic to, S288C


References:



Strathern et al.


(1979) Cell 18:309-319


DC5


Genotype:



MAT


a


leu2-3,112 his3-11,15 can1-11



Notes:


Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.


References:



Broach et al.


(1979) Gene 8:121-133


X2180-1A


Genotype:



MAT


a


SUC2 mal mel gal2 CUP1



Notes:


S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were


obtained from sporulated X2180


References:



Mortimer and Johnston



Sources:



ATCC:204504




YNN216


Genotype:



MAT


a/α



ura3-52/ura3-52 lys2-801


ambe r


/lys2-801


amber


a de2-101


ochre


/ade2-101


ochre



Notes:


Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).


References:



Sikorski RS and Hieter P


(1989) Genetics 122:19-27.


Sobel and Wolin


(1999) Mol. Biol. Cell 10:3849-3862.


YPH499


Genotype:



MAT


a


ura3-52 lys2-801_amber ade2-101_ochre trp1-


Δ


63 his3-


Δ


200 leu2-


Δ


1



Notes:


Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note


that


trp1-


Δ

63


, unlike


trp1-


Δ


1


, does not delete adjacent


GAL3


UAS sequence and retains homology to


TRP1


selectable


marker.


gal2-


, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis


1984; original source: M. Carlson, Columbia University), which is congenic with S288C.


References:



Sikorski RS and Hieter P


(1989) Genetics 122:19-27.


Sobel and Wolin


(1999) Mol. Biol. Cell 10:3849-3862.


Johnston M and Davis RW


(1984) Mol Cell Biol 4(8):1440-8.


Sources:



ATCC:204679



YPH500


Genotype:



MAT


α



ura3-52 lys2-801_amber ade2-101_ochre trp1-


Δ


63 his3-


Δ


200 leu2-


Δ


1



Notes:


MAT


α strain isogenic to



YPH499


except at mating type locus. Derived from the diploid strain YNN216


(Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.


References:



Sikorski RS and Hieter P


(1989) Genetics 122:19-27.


Sobel and Wolin


(1999) Mol. Biol. Cell 10:3849-3862.


Johnston M and Davis RW


(1984) Mol Cell Biol 4(8):1440-8.


Sources:



ATCC:204680



YPH501


Genotype:



MAT


a/


MAT

α



ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre


trp1-


Δ


63/trp1-


Δ


63 his3-


Δ


200/his3-


Δ


200 leu2-


Δ


1/leu 2-


Δ


1



Notes:



a/α diploid isogenic to



YPH499


and


YPH500


. Derived from the diploid strain YNN216 (Johnston and Davis


1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

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