-
Commonly used strains
This page
describes some of the most commonly used yeast lab
strains. Much of the information is taken from
F.
Sherman (2002)
Getting started with yeast, Methods Enzymol. 350,
3-41. Other useful papers for strain background
information include:
?
?
Mortimer and Johnston
(1986)
Genetics 113:35-43 - thoroughly describes the
genealogy of strain S288C
van Dijken et
al.
(2000) Enzyme Microb Technol
26:706-714 - compares various characteristics of
commonly
used lab strains
?
Winzeler et
al.
(2003) Genetics 163:79-89 - uses
SFP (single-feature polymorphisms) analysis to
study genetic
identity between common
lab strains
S288C
Genotype:
MAT
α
SUC2 gal2 mal mel flo1 flo8-1 hap1 ho
bio1 bio6
Notes:
Strain used in the systematic sequencing project,
the sequence stored in SGD. S288C does not form
pseudohyphae. In addition, since it has
a mutated copy of
HAP1
, it
is not a good strain for mitochondrial studies. It
has
an allelic variant of
MIP1
which increases petite
frequency. S288C strains are
gal
2- and they do not use
galactose
anaerobically.
The
S288C genome was recently resequenced at the
Sanger Institute
.
References:
Mortimer and Johnston
(1986)
Genetics 113:35-43.
Sources:
ATCC:204508
BY4743
Genotype:
MAT
a/α
his3
Δ
1/his3
Δ
1 leu2
Δ
0/leu2
< br>Δ
0 LYS2/lys2
Δ
0
met15
Δ
0/MET15 ura3
Δ
0/ura3
Δ
0
Notes:
Strain used in
the
systematic deletion
project
, generated from a cross between
BY4741 and BY4742, which
are derived
from S288C. As S288c, these strains have an
allelic variant of
MIP1
which increases petite frequency.
See
Brachmann et al. reference for details.
References:
Brachmann et al.
(1998)
Yeast 14:115-32.
Sources:
Biosystems:YSC1050
FY4
Genotype:
MAT
a
Notes:
Derived from S288C.
References:
Winston et al.
(1995) Yeast
11:53-55.
Brachmann et al.
(1998) Yeast 14:115-32.
FY1679
Genotype:
MAT
a/α
ura3-52/ura3-52
trp1
Δ
63/TRP1
leu2
Δ
1/LEU2
his3
Δ
200/HIS3
GAL2/GAL
Notes:
Isogenic to S288C; used in the systematic
sequencing project, the sequence stored in SGD.
References:
Winston et al.
(1995) Yeast
11:53-55.
Sources:
EUROSCARF:10000D
AB972
Genotype:
MAT
α
X2180-1B
trp1
0
[rho
0
]
Notes:
Isogenic to S288C;
used in the systematic sequencing project, the
sequence stored in SGD. AB972 is an
ethidium bromide-induced rho-
derivative of the strain
X2180-1B-
trp1
.
References:
Olson
MV et al.
(1986) Proc. Natl. Acad. Sci.
USA 83:7826-7830.
Sources:
ATCC:204511
A364A
Genotype:
MAT
a
ade1 ade2
ura1 his7 lys2 tyr1 gal1 SUC mal cup
BIO
Notes:
Used
in the systematic sequencing project, the sequence
stored in SGD.
References:
Hartwell
(1967) J.
Bacteriol. 93:1662-1670.
Sources:
ATCC:208526
XJ24-24a
Genotype:
MAT
a
ho HMa HMα
ade6 arg4
-17 trp1-1 tyr7-1
MAL2
Notes:
Derived from, but not isogenic to, S288C
References:
Strathern et al.
(1979) Cell
18:309-319
DC5
Genotype:
MAT
a
leu2-3,112
his3-11,15 can1-11
Notes:
Isogenic to S288C;
used in the systematic sequencing project, the
sequence stored in SGD.
References:
Broach et al.
(1979) Gene
8:121-133
X2180-1A
Genotype:
MAT
a
SUC2 mal mel
gal2 CUP1
Notes:
S288c spontaneously
diploidized to give rise to X2180. The haploid
segregants X2180-1a and X2180-1b were
obtained from sporulated X2180
References:
Mortimer and Johnston
Sources:
ATCC:204504
YNN216
Genotype:
MAT
a/α
ura3-52/ura3-52 lys2-801
ambe
r
/lys2-801
amber
a
de2-101
ochre
/ade2-101
ochre
Notes:
Congenic to S288C (see Sikorski and Hieter). Used
to derive YSS and CY strains (see Sobel and
Wolin).
References:
Sikorski RS and Hieter P
(1989) Genetics 122:19-27.
Sobel and
Wolin
(1999) Mol. Biol. Cell
10:3849-3862.
YPH499
Genotype:
MAT
a
ura3-52
lys2-801_amber ade2-101_ochre
trp1-
Δ
63
his3-
Δ
200
leu2-
Δ
1
Notes:
Contains
nonrevertible (deletion) auxotrophic mutations
that can be used for selection of vectors. Note
p>
that
trp1-
Δ
63
, unlike
trp1-
Δ
1
, does not delete
adjacent
GAL3
UAS sequence
and retains homology to
TRP1
selectable
marker.
gal2-
,
does not use galactose anaerobically. Derived from
the diploid strain YNN216 (Johnston and Davis
1984; original source: M. Carlson,
Columbia University), which is congenic with
S288C.
References:
Sikorski RS and Hieter P
(1989) Genetics 122:19-27.
Sobel and
Wolin
(1999) Mol. Biol. Cell
10:3849-3862.
Johnston M and Davis
RW
(1984) Mol Cell Biol 4(8):1440-8.
Sources:
ATCC:204679
YPH500
Genotype:
MAT
α
ura3-52 lys2-801_amber ade2-101_ochre
trp1-
Δ
63
his3-
Δ
200
leu2-
Δ
1
Notes:
MAT
α strain
isogenic to
YPH499
except at mating type
locus. Derived from the diploid strain YNN216
(Johnston and Davis 1984; original
source: M. Carlson, Columbia University), which is
congenic with S288C.
References:
Sikorski RS and Hieter P
(1989) Genetics 122:19-27.
Sobel and
Wolin
(1999) Mol. Biol. Cell
10:3849-3862.
Johnston M and Davis
RW
(1984) Mol Cell Biol 4(8):1440-8.
Sources:
ATCC:204680
YPH501
Genotype:
MAT
a/
MAT
α
ura3-52/ura3-52
lys2-801_amber/lys2-801_amber
ade2-101_ochre/ade2-101_ochre
trp1-
Δ
63/trp1-
Δ
63 his3-
Δ
200/his3-
Δ
200 leu2-
Δ
1/leu
2-
Δ
1
Notes:
a/α
diploid isogenic to
YPH499
and
YPH500
. Derived from the
diploid strain YNN216 (Johnston and Davis
1984; original source: M. Carlson,
Columbia University), which is congenic with
S288C.
-
-
-
-
-
-
-
-
-
上一篇:SAP-PP-代码大全
下一篇:常见英文人名大全,英文人名含义